Journal: Biological Procedures Online

Article Title: Computational Biology Methods and Their Application to the Comparative Genomics of Endocellular Symbiotic Bacteria of Insects

doi: 10.1007/s12575-009-9004-1

Figure Lengend Snippet: a Whole genome shotgun sequencing: Genome is sheared into small approximately equal sized fragments which are subsequently small enough to be sequenced in both directions followed by cloning . The cloned sequences (reads) are then fed to an assembler (illustrated in Figure 2). b To overcome some of the complexity of normal shotgun sequencing of large sequences such as genomes a hierarchical approach can be taken. The genome is broken into a series of large equal segments of known order which are then subject to shotgun sequencing. The assembly process here is simpler and less computationally expensive.

Article Snippet: In the race to publish the Human genome in the early 2000s, the Celera Whole Genome Assembler was engineered to accommodate large genomes.

Techniques: Shotgun Sequencing, Cloning, Clone Assay